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Modifying Enzymes

Proteins that have the ability to structurally alter macromolecules without being altered themselves. Useful for a variety of molecular biology procedures; products include DNA and RNA polymerases, phosphatases, kinases, nucleases, etc.
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Cytiva Ready-To-Go™ T4 DNA Ligase

For covalently linking DNA fragments in as little as 30 minutes

257

R&D Systems™ Caspase-6 Inhibitor Z-VEID-FMK
SDP

Used as cell-permeable, irreversible inhibitor of caspase activity. R&D Systems Caspase-6 Inhibitor Z-VEID-FMK binds to the active site of activated proteases irreversibly and inhibits apoptosis.

259

MP Biomedicals™ Sorbitol Dehydrogenase, From Sheep Liver

An oxidoreductase in carbohydrate metabolism converting fructose into sorbitol

Content And Storage 0°C
Form Powder
Product Type Sorbitol Dehydrogenase, L-Iditol dehydrogenase, Polyol dehydrogenase, SDH
261

R&D Systems™ Pan Caspase Inhibitor Z-VKD-FMK Biotinylated
SDP

Used as an intracellular detector of apoptotic cells. R&D Systems Caspase-13 Inhibitor Z-LEED-FMK binds to the active site of activated proteases irreversibly and inhibits apoptosis.

263

MP Biomedicals™ Cell Preparation Grade Collagenase from Clostridium histolyticum

Prepared to contain lower tryptic activity to limit damage to membrane proteins and receptors but with normal to above normal collagenase activity

Content And Storage Store at 4°C. Protect from moisture.
Form Powder
Color Brown
264

MP Biomedicals™ Collagenase Type III from Clostridium histolyticum

Collagenases degrades native helical collagen fibrils

Content And Storage 4°C
Inhibitors Metal chelating agents such as Cysteine, EDTA or o-Phenanthroline
Form Lyophilized Solid
Product Type Collagenase Type III
Storage Buffer 1mg/mL of Enzyme in 0.05M TES with 0.36mM Calcium Chloride, pH 7.5
Molecular Weight (g/mol) 105000 and 57400
Color Brown
pH 7 to 9
Concentration 1 mg/mL
For Use With (Application) Cell dissociation and cell lysis
Source Clostridium histolyticum
265

MilliporeSigma™ Calbiochem™ Y-27632

Y-27632A, CAS 146986-50-7, is a cell-permeable, reversible, inhibitor of Rho kinases (Ki = 140 nM for p160ROCK). Enhances survival & cloning efficiency of ESC without affecting their pluripotency.

Content And Storage Hygroscopic
Protect from Light
Form Solid
Molecular Weight (g/mol) 338.3g/mol
Color White
Purity 95% by HPLC
267

MP Biomedicals™ Catalase from Beef Liver

Oxidoreductase enzyme for catalyzing conversion of hydrogen peroxide to water and oxygen, 2H2O2 → 2H2O + O2

Content And Storage 0°C
Form Lyophilized Powder
Product Type Catalase
Molecular Weight (g/mol) 250
Color Dark Green
Concentration ≈13,000 U/mg
For Use With (Application) Used in the food industry for removing Hydrogen Peroxide from milk prior to cheese production
Source Beef liver
268

MP Biomedicals™ Creatinine Deiminase

>10u/mg solid, White Powder. Use to investigate the bioelectronic tongue for the simultaneous determination of urea, creatinine and alkaline ions in clinical samples.

Content And Storage -20°C
Form Lyophilized Powder
Product Type Creatinine Deiminase
Color White
pH 8.5 to 9.5
Concentration >10 U/mg
For Use With (Application) Determination of Urea, Creatinine and Alkaline Ions
Source Microbes